Retinal Gene Delivery by rAAV and DNA Electroporation
نویسندگان
چکیده
منابع مشابه
Retinal gene delivery by rAAV and DNA electroporation.
Ocular gene therapy is a fast-growing area of research. The eye is an ideal organ for gene therapy since it is immune privileged and easily accessible, and direct viral delivery results primarily in local infection. Because the eye is not a vital organ, mutations in eye-specific genes tend to be more common. To date, over 40 eye-specific genes have been identified harboring mutations that lead ...
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DNA vaccines have been demonstrated to be potent in small animals but are less effective in primates. One limiting factor may be inefficient uptake of DNA by cells in situ. In this study, we evaluated whether cellular uptake of DNA was a significant barrier to efficient transfection in vivo and subsequent induction of immune responses. For this purpose, we used the technique of electroporation ...
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Loree Heller and M. Lee Lucas Center for Molecular Delivery, University of South Florida, Tampa, FL 33612, Department of Medical Microbiology, University of South Florida, Tampa, FL 33612 __________________________________________________________________________________ * Correspondence : Loree Heller, University of South Florida, Center for Molecular Delivery, MDC16, 12901 Bruce B. Downs Blvd....
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ژورنال
عنوان ژورنال: Current Protocols in Microbiology
سال: 2013
ISSN: 1934-8525,1934-8533
DOI: 10.1002/9780471729259.mc14d04s28